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目的检测鼻咽癌组织中抑癌基因p16、p130/Rb2、nm23-H1基因在mRNA水平上的表达,探讨其与鼻咽癌诊断及其生物学行为的关系。方法p16基因mRNA检测采用生物素标记RNA探针的原位杂交技术;p130/Rb2、nm23-H1基因先提取鼻咽癌及鼻咽慢性炎症患者鼻咽部活检组织的总RNA,采用实时荧光定量RT-PCR技术检测鼻咽癌组织中p130和nm23基因mRNA含量。结果30例NPC标本原位杂交阳性13例,p16mRNA阳性表达为43.3%,3个高发家系的NPC标本无阳性表达,20例对照(鼻咽炎患者的标本)全部有p16mRNA阳性表达。鼻咽癌组p130和nm23-H1基因mRNA含量显著地低于对照组,经秩和检验P值<0.05,p130和nm23-H1基因mRNA在癌组织中表达与慢性咽炎组织比较均有显著性差异;鼻咽癌有无转移者其表达也有显著性差异。结论表明p16、p130/Rb2、nm23-H1mRNA在鼻咽癌组织中均呈低表达,提示p16、p130/Rb2、nm23-H1基因的突变或缺失可能对鼻咽癌的发生发展及恶性行为有重要意义。
Objective To detect the expression of tumor suppressor genes p16, p130 / Rb2 and nm23-H1 in nasopharyngeal carcinoma tissues and to investigate their relationship with the diagnosis and biological behavior of nasopharyngeal carcinoma. Methods The p16 gene mRNA was detected by in situ hybridization with biotin-labeled RNA probe. Total RNA was extracted from nasopharyngeal biopsies from patients with nasopharyngeal carcinoma and nasopharyngeal chronic inflammation by p130 / Rb2 and nm23-H1 genes. Real-time fluorescence quantitative The mRNA levels of p130 and nm23 in nasopharyngeal carcinoma tissues were detected by RT-PCR. Results Thirty-three NPC specimens were positive by in situ hybridization and the positive rate of p16 mRNA was 43.3%. No NPC specimens were found in the three high-risk families. All 20 NPC specimens were positive for p16 mRNA. The mRNA levels of p130 and nm23-H1 in nasopharyngeal carcinoma group were significantly lower than those in control group (P <0.05). The expressions of p130 and nm23-H1 mRNA in cancer tissue were significantly different from those in chronic pharyngitis There was also a significant difference in the expression of NPC with or without metastasis. The results showed that the low expression of p16, p130 / Rb2 and nm23-H1mRNA in nasopharyngeal carcinoma suggest that the mutation or deletion of p16, p130 / Rb2 and nm23-H1 may play an important role in the carcinogenesis and malignancy of nasopharyngeal carcinoma significance.