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目的探讨以甘露糖(mannose)修饰超顺磁纳米颗粒(superparamagnetic iron oxide nanoparticles,SPION)的制备方法并检测其理学性质,通过体外实验探讨该体系能否被网状内皮系统吞噬。方法采用共沉淀法制备Fe3O4纳米颗粒,然后加入甘露糖溶液,在通氮条件下90℃剧烈搅拌2 h,得到甘露糖包被的SPION。透射电镜测量其大小和分布,傅里叶红外光谱判断甘露糖修饰效果,采用原子吸收分光光度计检测样品中铁含量,体外白细胞吞噬实验检测其能否被白细胞吞噬,细胞毒性实验检测样品的安全性。结果所得样品为球形或类球形,分散性好,核心为Fe3O4晶体,核心粒径为(12.89±3.29)nm,包被甘露糖后的整体颗粒直径不超过20 nm;样品的铁含量为69.84 mg/L;红外吸收光谱测定结果表明甘露糖成功结合到Fe3O4的表面;体外白细胞吞噬实验表明白细胞对甘露糖修饰的SPION的吞噬率较高,细胞毒性实验表明其对细胞的IC50为100.44μg/ml。结论制备的mannose-SPION具有粒径小、分散性好、超顺磁性等优点,容易被吞噬细胞吞噬,且细胞毒性较低。
OBJECTIVE: To investigate the preparation method of mannose-modified superparamagnetic iron oxide nanoparticles (SPION) and to examine the physical properties of mannan-based superparamagnetic iron oxide nanoparticles (SPION). In vitro experiments were conducted to investigate whether this system can be phagocytosed by reticuloendothelial system. Methods Fe3O4 nanoparticles were prepared by coprecipitation method, then mannose solution was added and stirred vigorously at 90 ℃ for 2 h under nitrogen atmosphere to obtain mannose-coated SPION. Transmission electron microscopy was used to measure the size and distribution of mannose. Fourier transform infrared spectroscopy (FTIR) was used to determine the effect of mannose modification. Atomic absorption spectrophotometer was used to detect the content of iron in the sample. In vitro leukocyte phagocytosis test was used to detect whether the mannose was phagocytosed. Cytotoxicity test . Results The samples were spherical or spheroidal with good dispersity. The core was Fe3O4 crystal with the core diameter of (12.89 ± 3.29) nm. The total particle size of the coated mannose particles did not exceed 20 nm. The iron content of the sample was 69.84 mg / L. The results of infrared absorption spectroscopy showed that mannose was successfully bound to the surface of Fe3O4. In vitro leukocyte phagocytosis experiments showed that phagocytosis of mannose-modified SPION by leukocytes was higher. Cytotoxicity assay showed that the IC50 of the cells was 100.44μg / ml . Conclusion The prepared mannose-SPION has the advantages of small particle size, good dispersibility and superparamagnetism, is easily phagocytosed by phagocytes and has low cytotoxicity.