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目的探索日本血吸虫尾蚴标本制作流程的改进。方法启用灿烂甲酚兰、伊红染色,改革制作流程,省略了传统制作中的固定、分色、脱水、透明等步骤。结果制成的日本血吸虫尾蚴标本,结构清晰美观,虫体弯曲自然,无卷曲、重叠。结论改进后的方法效果好,提高了标本的制作质量,而且操作简单易行,值得在有关教学与科研单位推广。
Objective To explore the improvement of the production process of Schistosoma japonicum cercariae specimen. Methods The process of brilliant cresol blue and eosin staining was enabled and the production process was reformed. The steps of fixation, color separation, dehydration and transparency in traditional production were omitted. Results made of Schistosoma japonicum cercariae specimens, clear and beautiful structure, body bending natural, non-curly, overlapping. Conclusion The improved method is effective and improves the quality of specimen preparation. Moreover, it is easy to operate and worthy of promotion in relevant teaching and research institutes.