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用ＨＲＰ注入孤束核逆行追踪和５－羟色胺（５－ＨＴ）免疫组织化学相结合的双标记法，观察大鼠孤束核内的５－ＨＴ能纤维和终末的来源。结果在脑干内见到许多ＨＲＰ单标、５－ＨＴ单标和ＨＲＰ／５－ＨＴ双标细胞，其中ＨＲＰ／５－ＨＴ双标细胞主要分布于脑桥被盖核（２６．５７％）、脑桥网状核（２４．１９％）、中缝大核（２１．１７％），其次为中缝隐核（１２．７４％）、中缝苍白核（７．７８％），少数见于中缝背核、中脑导水管周围灰质和中缝脑桥核等处。而在结状神经节内仅见到ＨＲＰ单标细胞，未见到５－ＨＴ单标和ＨＲＰ／５－ＨＴ双标细胞。以上结果表明，大鼠孤束核的５－ＨＴ能纤维和终末主要来源于脑桥与延髓的５－ＨＴ神经元的投射。本研究提示脑桥与延髓内上述核团的５－ＨＴ神经元通过向孤束核的直接投射参与孤束核内脏功能的调控。
The HRP-injected nucleus retrograde tracing and 5-HT (5-HT) immunohistochemistry were used to detect the origin of 5-HT fibers and terminals in the nucleus tractus solitarius. Results HRP / 5-HT double-labeled cells were mainly found in the brainstem nucleus (26.57%), many single HRP, 5-HT and HRP / 5- The pontine reticular nucleus (24.19%) and the large central stapes (21.17%) were followed by latent stapes (12.74%) and pale stained nuclei (7.78%). A few were found in the dorsal raphe, Cerebral aqueduct around the gray matter and the middle cerebral pontine nucleus and so on. Only HRP single-labeled cells were seen in the nodose ganglion, and 5-HT single-labeled and HRP / 5-HT double-labeled cells were not seen. The above results show that the 5-HT fibers and terminals of the nucleus tractus solitarius mainly originate from the projection of 5-HT neurons in the pons and medulla. The present study suggests that 5-HT neurons in the pontine and medulla oblongata are involved in the regulation of visceral function of the solitary tract by direct projection to the nucleus tractus solitarius.