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目的确定引起2001年江苏省徐州地区无菌性脑膜炎流行的病原体。方法组织培养法从患者脑脊液分离病毒,标准血清中和试验鉴定分离毒株;中和试验检测双份血清中和抗体效价;逆转录聚合酶链反应(RT-PCR)检测肠道病毒特异性基因片段。结果22份脑脊液中分离出4株柯萨奇B5型、2株柯萨奇B3型、1株艾可7型肠道病毒,分离阳性率31.8%。RT-PCR检测脑脊液21份,肠道病毒阳性11份,阳性率52.4%。19例双份血清中11例中和效价呈4倍以上增长或转阳,阳性率57.9%。结论此次江苏省徐州地区无菌性脑膜炎流行的病原体是以柯萨奇B5为主要血清型的肠道病毒。
Objective To determine the causative agent of aseptic meningitis in Xuzhou, Jiangsu Province in 2001. Methods Tissue culture method was used to isolate virus from patients with cerebrospinal fluid (CSF). The neutralizing test was used to detect the neutralizing antibody titers of the two serum samples. RT-PCR was used to detect the specificity of enteroviruses Gene fragment. Results Four strains of Coxsackie B5, two Coxsackie B3 and one AIK 7 were isolated from 22 cerebrospinal fluid. The positive rate was 31.8%. 21 samples of cerebrospinal fluid (CSF) were detected by RT-PCR and 11 were positive by enterovirus. The positive rate was 52.4%. Neutralization titer in 11 cases of double serum in 19 cases was more than 4 times increase or turn positive, the positive rate was 57.9%. Conclusion The pathogen of aseptic meningitis epidemic in Xuzhou, Jiangsu Province is enterovirus which is mainly serotype Coxsackie B5.