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目的探讨酒精诱发乳腺癌的分子机制。方法采用Realtime PCR方法分析酒精对雌激素受体α(ERα)不同表达状况的乳腺细胞系、酒精联合雌激素MCF-7细胞系以及酒精对转染ERαsiRNA的MCF-7细胞系的RNA聚合酶Ⅲ依赖基因(tRNAs、5S rRNA)的转录水平。结果酒精对ER+乳腺细胞系tRNAs、5S rRNA上调水平(7~8倍)明显高于ER-乳腺细胞系(2~3倍)(P<0.01);酒精和雌激素联合处理对MCF-7细胞系tRNAs、5S rRNA的转录水平的上调作用(12~14倍)明显高于酒精(7~8倍)或雌激素(2~3倍)单独作用组(P<0.01);酒精对转染ERαsiRNA组MCF-7细胞系(2~3倍)tRNAs、5S rRNA的转录水平的上调作用明显低于未转染组(6~8倍)(P<0.01)。结论酒精诱发乳腺癌过程中RNA聚合酶Ⅲ依赖基因转录水平的上调依赖于ERα的表达。
Objective To investigate the molecular mechanism of alcohol induced breast cancer. Methods Realtime PCR was used to analyze the expression of estrogen receptor α (ERα) in breast cancer cell line, alcohol combined with estrogen MCF-7 cell line and alcohol transfected ERαsiRNA MCF-7 cell line RNA polymerase Ⅲ Dependent gene (tRNAs, 5S rRNA) transcription level. Results Alcohol up-regulated the mRNA and 5S rRNA levels of ER and mammary gland cell lines (7-8 fold) compared with ER-mammary gland cell lines (2-3 fold) (P <0.01) (12- to 14-fold) were significantly higher than that of ethanol (7-8-fold) or estrogen (2-3-fold) alone (P <0.01) The upregulation of tRNAs and 5S rRNA in group MCF-7 (2-3 fold) was significantly lower than that in untransfected group (P <0.01). Conclusion The up-regulation of RNA polymerase Ⅲ-dependent gene transcription in alcohol-induced breast cancer depends on the expression of ERα.