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目的以日本血吸虫感染猪为模型,研究不同感染状态下针对糖基产生的抗体在宿主免疫应答中的作用。方法建立日本血吸虫感染猪模型,分别检测不同感染状态下猪血清中针对LNFPIII、N-糖苷水解酶F(PNGaseF)处理可溶性虫卵抗原(SEA)前后及过碘酸处理SEA前后的抗体水平。结果辐照致弱尾蚴单纯免疫组、正常尾蚴单纯感染组和免疫后予以感染的免疫攻击组的猪均针对LNFPIII产生了相应的IgM抗体应答。3组猪血清中针对PNGaseF处理SEA的特异性抗体IgG、IgM水平较未处理组略低,但抗体IgG和IgM水平差异均无统计学意义(P均>0.05)。在单纯免疫组,猪血清中针对过碘酸处理SEA的特异性IgG、IgM、IgA抗体水平均较未处理组有所下降,差异有统计学意义(P均<0.01),但辐照致弱日本血吸虫糖基成分所诱导的抗体水平均较低。在单纯感染组,猪血清中针对过碘酸处理SEA的抗体IgG、IgM水平在感染后第8周下降极为显著,抗体水平差异有统计学意义(P均<0.01),其中糖基所诱导的抗体亚型以IgG1为主。此时,在虫负荷显著降低的免疫攻击组,日本血吸虫糖基成分能够显著增强IgG、IgM、IgA,特别是IgG2的表达。结论日本血吸虫糖基成分能够诱导宿主产生多种类型的抗体应答;且抗体亚型的表达与宿主的感染状态相关,即IgG1亚型的表达与日本血吸虫急性感染期的虫负荷和卵负荷一致;而具有免疫保护力的宿主能够针对日本血吸虫糖基成分产生IgG2应答。
OBJECTIVE: To study the role of antibodies against glycosylation in host immune response under different infection status in Schistosoma japonicum-infected pigs. Methods The pigs infected with Schistosoma japonicum were established to detect the levels of antibodies in serum of pigs before and after SEA treatment with LNFPIII, PNGaseF and SEA respectively. Results The pigs immunized with weak cercariae alone, normal cercaria larvae alone and those immunized with immunization challenged with LNFPIII. The serum IgG and IgM levels of SEA against PNGaseF were lower than those of untreated group, but there was no significant difference of IgG and IgM between the three groups (P> 0.05). The levels of specific IgG, IgM and IgA antibodies to SEA in the simple immunization group and the pig serum were all significantly lower than those in the untreated group (P <0.01), but the irradiation was weak Schistosoma japonicum glycosylation components induced by the antibody levels were low. Antibody IgG and IgM levels in SEA-infected SEA-infected pigs in the simple infection group were significantly decreased at the 8th week after infection, with significant differences in antibody levels (all P <0.01), among which glyco-induced The antibody subtype is mainly IgG1. At this time, in the immune attack group in which the worm burden was significantly reduced, the Schistosoma japonicum glycosylation components significantly enhanced the expression of IgG, IgM, and IgA, especially IgG2. Conclusion The glycoconjugation of Schistosoma japonicum could induce many kinds of antibody responses in host, and the expression of antibody subtypes was correlated with the infection status of hosts. The expression of IgG1 subtype was consistent with the insect burden and egg load in acute infection stage of Schistosoma japonicum. While the immunoprotective host is able to produce an IgG2 response against S. japonicum glycosyl components.