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用明胶将辣根过氧化物酶(HRP)固定于多壁碳纳米管(MWNT)和茜素红(AR)修饰的玻碳(GC)电极上,制成HRP生物传感器(HRP/AR/MWNT/GC),然后在3%戊二醛(GA)中进行交联改性,以克服明胶膜易溶胀的缺点,并提高膜的稳定性.同时详细探讨了该传感器对H2O2的响应性能,并优化了实验条件.结果表明,该传感器对H2O2的线性响应范围为5.0×10-6～1.0×10-3mol/L,线性相关系数为0.9932,检出限为1.0×10-7mol/L,且放于4℃环境30d后,峰电流值约为原来的72.1%.该传感器响应快速,灵敏度高,且具有良好的重现性、稳定性及较长的使用寿命,具有潜在的应用价值.
Horseradish peroxidase (HRP) was immobilized on multi-walled carbon nanotubes (MWNT) and alizarin red (AR) modified glassy carbon (GC) electrodes with gelatin to make HRP / AR / MWNT / GC), and then modified by 3% glutaraldehyde (GA) to overcome the shortcoming of gelatin swelling and improve the stability of the membrane.At the same time, the sensor response to H2O2 was discussed in detail The experimental conditions were optimized.The results showed that the linear response range of the sensor to H2O2 was 5.0 × 10-6 ~ 1.0 × 10-3mol / L, the linear correlation coefficient was 0.9932, the detection limit was 1.0 × 10-7mol / L, and After placed in 4 ℃ environment for 30 days, the peak current value is about 72.1% of the original.The sensor has the advantages of fast response, high sensitivity and good reproducibility, stability and long service life, and has potential application value.