鲎试验已广泛应用于内毒素检测,但近几年来发现常规鲎试剂对内毒素反应并不专一,它受其中含有的G因子干扰。采用亲和层析法分离鲎血细胞崩解液中的凝固酶原和B、C因子,进行重组实验,制得一种敏感专一的鲎BC因子试剂,本文概述其制备和测定方法。 鲎 Tests have been widely used in endotoxin testing, but in recent years it has been found that conventional 鲎 reagents are not specific to endotoxin responses and are disturbed by factor G contained therein. Affinity chromatography method was used to separate the coagulase and B and C factors in the blood cell disintegration fluid and to carry out recombination experiments to obtain a sensitive and specific BCF reagent. The preparation and determination methods of this method were summarized in this paper.