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目的:研究桑枝多糖对链脲佐菌素(STZ)诱导糖尿病大鼠的血糖和抗氧化作用的影响。方法:STZ(120 mg·kg~(-1))腹腔注射诱导糖尿病大鼠模型,并随机分为5个组(n=10):模型对照组,二甲双胍对照组(320 mg·kg~(-1)),桑枝多糖低、中、高剂量组(0.3,0.6,1.2 g·kg~(-1)),另设10只大鼠作为空白对照组。大鼠灌胃给药60 d,并测定大鼠空腹血糖(FBG)。终点法测24h尿微量白蛋白含量。给药60 d后处死大鼠,对大鼠肾脏HE染,光镜下观察肾脏病理学的变化;试剂盒测定大鼠肾脏中锰超氧化物歧化酶(Mn-SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性和丙二醛(MDA)含量;比色法检测肾组织中线粒体呼吸链复合物Ⅰ、Ⅲ活性。结果:与模型组比较,糖尿病大鼠经桑枝多糖治疗后血糖有所下降并显著降低尿微量白蛋白含量(P<0.05)。模型组大鼠肾脏中Mn-SOD、GSH-Px活性以及肾组织线粒体呼吸链复合物Ⅰ、Ⅲ活性显著降低,而MDA含量则升高。桑枝多糖给药后Mn-SOD、GSH-Px、线粒体呼吸链复合物Ⅰ、Ⅲ活性有所升高,MDA含量下降(P<0.05)。结论:桑枝多糖能显著降低糖尿病大鼠的血糖,其作用机制可能与提高糖尿病大鼠的抗氧化作用有关。
Objective: To study the effects of mulberry polysaccharides on blood glucose and antioxidation in streptozotocin (STZ) -induced diabetic rats. Methods: Diabetic rat models were induced by intraperitoneal injection of STZ (120 mg · kg -1) and were randomly divided into 5 groups (n = 10): model control group, metformin control group (320 mg · kg -1) 1)), mulberry polysaccharide low, medium and high dose groups (0.3,0.6,1.2 g · kg -1), and another 10 rats served as blank control group. Rats were intragastrically administered for 60 days, and fasting blood glucose (FBG) was measured in rats. End point measured 24h urine microalbumin content. Rats were sacrificed 60 d after administration, HE staining of rat kidneys, pathological changes of kidneys were observed under light microscope. The contents of Mn-SOD and glutathione (GSH-Px) activity and malondialdehyde (MDA) content were detected by enzyme-linked immunosorbent assay (ELISA). The mitochondrial respiratory chain complex I and III activities in renal tissue were detected by colorimetric assay. Results: Compared with the model group, the blood glucose of diabetic rats decreased and the content of urine microalbumin significantly decreased (P <0.05). The activities of Mn-SOD and GSH-Px in the kidney of rats in the model group were significantly lower than those in the control group (P <0.05). The activities of Mn-SOD, GSH-Px, mitochondrial respiratory chain complex I and III increased after administration of mulberry polysaccharide, and the content of MDA decreased (P <0.05). Conclusion: Mulberry polysaccharide can significantly reduce the blood sugar in diabetic rats, and its mechanism may be related to improving antioxidant function of diabetic rats.