13对输精管先天缺如的不育夫妇作显微外科附睾精子抽吸和体外受精(IVF),共有104个胚用作冷冻保存。精子抽吸和IVF按Silber的方法,冷冻和解冻按Testart的方法。胚转送到输卵管(若为通畅)或转送到子宫。所有冷冻胚的替代周期中,从上次月经周期的第21天起使用一种促性腺释放激素拮抗剂乙酸Leuprolide作垂体抑制,每天1mg,直至胚转送的前3～4天。自月经期的第3天起,每日服2mg17β-雌二醇作 Thirteen sterile embryos with vas deferens birth defects were used for microsurgical epididymal sperm aspiration and in vitro fertilization (IVF). A total of 104 embryos were used for cryopreservation. Sperm aspiration and IVF by the Silber method, frozen and thawed according to the Testart method. The embryos are transferred to the fallopian tubes (if unobstructed) or to the uterus. In all surrogate cycles of frozen embryos, pituitary inhibition with a gonadotrophin-releasing hormone antagonist, acetic acid Leuprolide, was started on the 21st day of the last menstrual cycle from 1 mg daily until the first 3 to 4 days of embryo transfer. From day 3 of the menstrual period, 2 mg of 17β-estradiol was administered daily