作为酶促催化底物之一的2,6-二甲氧基苯酚(DMP)经常用于测定漆酶活性.然而到目前为止,其酶促产物结构却仍不清楚.由此,研究了漆树漆酶对其催化氧化反应.在水/有机溶剂体系中,催化得到一种产物3,3′,5,5′-四甲氧基-1,1′-联苯-4-4′-二酚(TMBP),对其用红外、核磁和色谱-质谱等系统测定,并简要探讨了酶促反应机理. 2,6-dimethoxyphenol (DMP), one of the substrates for enzymatic catalysis, is often used to determine laccase activity. However, the structure of its enzymatic product remains unclear so far. Thus, Laccase on its catalytic oxidation.In the water / organic solvent system, a product of 3,3 ’, 5,5’-tetramethoxy-1,1’-biphenyl-4’4-bis Phenol (TMBP) was determined by infrared, nuclear magnetic resonance and mass spectrometry. The mechanism of enzymatic reaction was briefly discussed.