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目的:利用显微定量法,对赤芍簇晶进行显微定量研究;对赤芍显微特征常数进行测定研究。方法:1容量分析法:将药材粉碎,过筛,精称定量的粉末,放入干净的研钵中,用水合氯醛试液将粉末转移至25 m L容量瓶,加入相应的甘油量,用水合氯醛定容,得供试品混悬液。充分摇匀后,用微量移液器准确吸取0.02 m L样品液置载玻片上,盖上盖玻片,完成制片。在显微镜下按“之”字形观察,记录每张片子的显微特征个数,每个样品平行做3次,每次平行装片50张,将所得结果随机分为5组,计算每组的平均值,再按照公式计算显微特征常数值。2成药前处理方法:将成药小心粉碎至完全过一定目数的筛,加适量水,充分研磨搅匀,离心,倾出上层液,向沉淀中加入清水,将沉淀搅起与水混匀,离心,不断重复上述操作,直至上层液澄清,将沉淀取出,置烘箱内低温干燥(<60℃),待干透后再将其研磨过相同目数的筛,备用。
OBJECTIVE: To study the microscopic quantitative analysis of red peony cluster by microscopic quantitative method and to study the microscopic characteristic constant of red peony root. Methods: 1 Volumetric analysis: The herbs were crushed, sieved, accurately weighed powder, placed in a clean mortar, the chloral hydrate test solution with the powder transferred to a 25 mL volumetric flask, add the appropriate amount of glycerol, With chloral hydrate fixed volume, for the test suspension. Shake well and use a micropipette to accurately draw 0.02 mL sample solution onto a glass slide and cover with a coverslip to complete the filming. Under the microscope, press the “” shape observation, record the number of microscopic features of each film, each sample to do 3 times in parallel, each parallel loading 50, the results were randomly divided into 5 groups, each calculated The average value of the group, and then calculate the microscopic characteristic constant value according to the formula. 2 medicine pre-treatment methods: the medicine carefully crushed to completely over a certain number of mesh sieve, add appropriate amount of water, mix thoroughly grinding, centrifugation, pour out the upper liquid, add clear water to the precipitate, the precipitate stirred up with water, Centrifugal, repeat the above operation until the supernatant fluid is clear, the precipitate removed, set the oven low temperature drying (<60 ℃), to be dried and then grind the same mesh screen, spare.