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目的探讨热籽感应加热对体外培养的恶性黑色素瘤细胞的杀伤效果。方法将体外培养的B16恶性黑色素瘤细胞分为对照组、单独磁场组、单独热籽组(一颗热籽、二颗热籽)、热籽加温组(一颗热籽、二颗热籽)6组,其中对照组、热籽加温组又分为6个时间亚组(5、10、15、20、25、30min),各组分别处理后,倒置显微镜进行形态学观察,四甲基噻唑兰比色法测定细胞活性,得出每组处理后的细胞存活率。结果实验中所用的铁磁热籽在现有参数交变磁场中具有良好的升温效果,一颗热籽培养皿内加热温度可达(53.1±0.5)℃,二颗热籽则可达(56.5±0.5)℃。热籽感应加温对体外培养的B16细胞具有明显的杀伤效应,镜下观察出现明显的凋亡或坏死的形态学改变。二颗热籽加温组20min细胞存活率为(3.1±2.9)%,25min和30min便可将培养皿内肿瘤细胞全部杀死。加热30min时,单独磁场和单独热籽对B16细胞存活率影响不大,存活率分别为(98.1±5.1)%、(99.5±2.3)%、(94.6±11.0)%,与对照组相比均无统计学差异(分别为q对照-单独磁场=0.497,P>0.05;q对照-一颗热籽=0.120,P>0.05;q对照-二颗热籽=1.419,P>0.05;q单独磁场-一颗热籽=0.377,P>0.05;q单独磁场-二颗热籽=0.922,P>0.05;q一颗热籽-二颗热籽=1.299,P>0.05);加热30min时,热籽加温组和对照组、单独磁场组、单独热籽组存活率相比有统计学差异(q对照-一颗热籽加温=16.934,P<0.01;q单独磁场-一颗热籽加温=16.437,P<0.01;q一颗热籽-一颗热籽加温=16.814,P<0.01;q二颗热籽-一颗热籽加温=15.515,P<0.01)。结论热籽感应加温交变磁场中可升温到适宜的温度,有效杀伤体外培养的黑色素瘤细胞,导致肿瘤细胞凋亡或死亡。
Objective To investigate the killing effect of heat-induced induction on malignant melanoma cells cultured in vitro. Methods B16 malignant melanoma cells cultured in vitro were divided into control group, single magnetic field group, single hot seed group (one hot seed and two hot seeds), hot seed heating group (one hot seed and two hot seeds ) 6 groups, including the control group, hot seed heating group is divided into 6 time subgroups (5,10,15,20,25,30min), each group were treated, the inverted microscope for morphological observation, The cell viability was measured by the method of thiazolyl colorimetry and the cell viability after each treatment was obtained. Results The ferromagnetic seeds used in the experiment had a good heating effect in the existing parameters of the alternating magnetic field. The heating temperature in a heat-seeding dish reached 53.1 ± 0.5 ℃ and the two heat seeds reached 56.5 ± 0.5) ° C. Heat-induced induction of warm seedlings cultured in vitro B16 cells have obvious killing effect, microscopic observation showed obvious morphological changes of apoptosis or necrosis. The survival rate of the two heat-treated groups at 20min was (3.1 ± 2.9)%, and all the tumor cells in the culture dish were completely killed at 25min and 30min. When heated for 30 min, the survival rate of B16 cells was not significantly affected by magnetic field alone and heat seeds alone, the survival rates were (98.1 ± 5.1)%, (99.5 ± 2.3)% and (94.6 ± 11.0)%, respectively Q control - one hot seed = 0.120, P> 0.05; q control - two hot seeds = 1.419, P> 0.05; q independent magnetic field - one hot seed = 0.377, P> 0.05; q single magnetic field - two hot seeds = 0.922, P> 0.05; q one hot seed - two hot seeds = 1.299, P> 0.05) There was a significant difference in the survival rates between the seed heating group and the control group, the single magnetic field group and the single hot seed group (q control - one hot seed heating = 16.934, P <0.01; q single magnetic field - one hot seed plus Temperature = 16.437, P <0.01; q one hot seed - one hot seed heating = 16.814, P <0.01; q two hot seeds - one hot seed heating = 15.515, P <0.01). Conclusion Heat seeds induction heating alternating magnetic field can be warmed to the appropriate temperature, effectively killing cultured melanoma cells, leading to tumor cell apoptosis or death.