论文部分内容阅读
目的:寻找肺鳞状细胞癌组织中与正常组织中差异表达的微小RNA(miRNA),并对其进行生物学功能的预测及验证。方法:应用芯片技术筛选30例肺鳞癌组织中与正常组织中差异表达的miRNA。选取差异表达的mir-182家族进行定量PCR验证,通过生物信息学网站预测其靶基因,并在体外进行荧光素酶报告基因验证。结果:Hsa-mir-182家族在肺鳞癌组织中表达丰度较正常组织明显升高。生物信息学网站预测发现,RASA1可能是mir-182的靶基因。体外过表达mir-182后,发现RASA1表达量显著下降,验证了RASA1确实为mir-182的靶基因。结论:Hsa-mir-182家族在肺鳞癌组织中显著高表达,而且RASA1基因系mir-182的靶基因。
Objective: To find differentially expressed microRNAs (miRNAs) in squamous cell lung cancer tissues and normal tissues, and to predict and validate their biological functions. Methods: The miRNAs differentially expressed in 30 squamous cell carcinomas and normal tissues were screened by microarray technique. The differentially expressed mir-182 family was selected for quantitative PCR validation. The target genes were predicted by the bioinformatics website and verified by luciferase reporter assay in vitro. Results: The abundance of Hsa-mir-182 family in lung squamous cell carcinoma tissues was significantly higher than that in normal tissues. Bioinformatics website predicts that RASA1 may be the target gene of mir-182. After overexpression of mir-182 in vitro, RASA1 expression was significantly decreased, confirming that RASA1 is indeed the target gene of mir-182. Conclusion: The Hsa-mir-182 family is highly expressed in squamous cell carcinoma of the lung, and the RASA1 gene is the target gene of mir-182.