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目的改进和优化婴幼儿食品和乳品中维生素B6测定方法。方法样品经热水提取等前处理后,经C18柱分离,荧光检测器检测,外标法定量分析维生素B6(吡哆醇、吡哆醛和吡哆胺)的含量。结果增加甲醇比例至20%,维生素B6的3个组分出峰时间缩短为15 min。维生素B6在0~1.0μg/ml具有良好的线性,相关系数达0.999以上,检出限为吡哆醇1.5μg/100 g,吡哆醛1.5μg/100 g,吡哆胺1.5μg/100 g。方法的回收率为85%~110%之间,RSD小于5.0%。结论通过调整流动相比例,大大缩短了检测时间,节省了检测试剂,对环境污染减轻,且分离效果很好。
Objective To improve and optimize the determination of vitamin B6 in infant food and dairy products. Methods The samples were pretreated by hot water extraction, separated by C18 column and detected by fluorescence detector. The contents of vitamin B6 (pyridoxine, pyridoxal and pyridoxamine) were quantitatively analyzed by external standard method. The results of increasing the proportion of methanol to 20%, vitamin B6 3 component peak time was shortened to 15 min. Vitamin B6 has a good linearity at 0 ~ 1.0μg / ml, the correlation coefficient is above 0.999, the detection limits are 1.5μg / 100g of pyridoxine, 1.5μg / 100g of pyridoxal and 1.5μg / 100g of pyridoxamine . The recoveries of the methods ranged from 85% to 110% with RSDs less than 5.0%. Conclusion By adjusting the mobile phase ratio, the detection time is greatly shortened, the detection reagent is saved, the environmental pollution is relieved, and the separation effect is very good.