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目的了解广西公共场所集中式中央空调冷却塔水嗜肺军团菌污染状况,为科学防制提供依据。方法采集2009~2012年广西14个地级市公共场所301份集中式中央空调冷却塔水进行嗜肺军团菌培养及鉴定。结果共检出嗜肺军团菌95株,阳性率31.56%(95/301),LP1型占54.74%(52/95),LP2-15型占45.26%(43/95)。中央空调系统使用单位(酒店、宾馆、超市和医院)该菌阳性率40.29%(83/206)。45株嗜肺军团菌1型经脉冲场凝胶电泳(PFGE)分型,获得了41种基因型别,带型比较分散,未发现绝对优势带型。结论广西公共场所水体嗜肺军团菌污染普遍存在且较严重,对人群健康构成潜在威胁,需要引起重视,以防军团菌病的暴发流行。
Objective To understand the status of Legionella pneumophila contamination in centralized central air conditioning cooling tower in public places in Guangxi and provide the basis for scientific prevention and control. Methods A total of 301 central air-conditioner cooling tower water in 14 prefecture-level cities in Guangxi from 2009 to 2012 were collected for Legionella pneumophila culture and identification. Results A total of 95 Legionella pneumophila strains were detected, the positive rate was 31.56% (95/301), LP1 type accounted for 54.74% (52/95) and LP2-15 type accounted for 45.26% (43/95). The use of central air conditioning system units (hotels, guesthouses, supermarkets and hospitals) the positive rate of the bacteria 40.29% (83/206). Forty-five strains of Legionella pneumophila1 were genotyped by pulsed-field gel electrophoresis (PFGE), and 41 genotypes were obtained. The bands were scattered and no absolute predominant bands were found. Conclusion Legionella pneumophila pollution is widespread and serious in public places in Guangxi. It poses a potential threat to the health of the population and needs to be taken seriously to prevent the outbreak of Legionella.