论文部分内容阅读
通过观察烹调油烟冷凝物（ＲＣ）对大鼠肺组织超氧化物岐化酶（ＳＯＤ）和脂质过氧化产物丙二醛（ＭＤＡ）及骨髓微核（ＭＮ）率的变化，探讨了其变化规律和作用机理。采用ＲＣ气管灌注大鼠的方法，对ＲＣ的ＳＯＤ、ＭＤＡ、ＳＯＤ／ＭＤＡ比值及ＭＮ进行了分析。结果显示：低（２２５ｍｇ／ｋｇ）、中（４５０ｍｇ／ｋｇ）、高（９００ｍｇ／ｋｇ）３个剂量ＲＣ组和阳性苯并（ａ）芘［Ｂ（ａ）Ｐ］对照组大鼠肺组织ＳＯＤ活性明显低于溶剂二甲基亚砜（ＤＭＳＯ）组和阴性对照组（Ｐ＜０．０５），而且呈剂量－效应关系；３个剂量ＲＣ组和Ｂ（ａ）Ｐ组ＭＤＡ浓度明显高于ＤＭＳＯ和阴性对照组（Ｐ＜０．０５）；ＳＯＤ／ＭＤＡ比值均有下降趋势；中剂量ＲＣ使ＭＮ率上升，高剂量ＲＣ对ＭＮ率的效应更明显（Ｐ＜０．０１）。提示ＲＣ可使体内抗氧化和脂质过氧化平衡失调。表明ＲＣ对超氧化物歧化酶系统有强烈的抑制作用。长期接触烹调油烟对肺组织有明显损伤作用，甚至有可能诱发肺部肿瘤
By observing the change of the cooking fume condensate (RC) on the superoxide dismutase (SOD) and malondialdehyde (MDA) and bone marrow micronucleus (MN) rates in rat lung tissue, the changes were explored. Law and mechanism of action. Using RC tracheal perfusion in rats, the SOD, MDA, SOD/MDA ratio and MN of RC were analyzed. The results showed that SOD in lungs of rats with low (225 mg/kg), medium (450 mg/kg), high (900 mg/kg) 3 dose RC and positive benzo(a)pyrene [B(a)P] control groups The activity was significantly lower than the solvent dimethyl sulfoxide (DMSO) group and the negative control group (P<0.05), and showed a dose-effect relationship; the MDA concentrations of the three doses of RC group and B(a)P group were significantly higher DMSO and negative control group (P<0.05); SOD/MDA ratio had a decreasing trend; middle dose RC increased MN rate, and high dose RC had more significant effect on MN rate (P<0.01). It is suggested that RC can make the balance of antioxidation and lipid peroxidation in the body. This shows that RC has a strong inhibitory effect on the superoxide dismutase system. Long-term exposure to cooking fumes has significant damage to lung tissue and may even induce lung tumors