论文部分内容阅读
为确定内蒙古地区羊源大肠杆菌的耐药表型及其耐药基因的流行情况,本研究采用微量稀释法测定了内蒙古地区108株羊源大肠杆菌对13种临床常用抗菌药物的最小抑菌浓度。结果显示,分离菌株对阿莫西林、头孢噻吩、磺胺甲唑、黏菌素的耐药率最高,均达100.0%,对阿莫西林/克拉维酸、四环素、环丙沙星的耐药率在50%~80%之间,对头孢噻肟、美洛培南、新霉素的耐药率均低于10%,较为敏感。108株羊源大肠杆菌中耐7种以上药物的菌株占94.4%,其中15.6%菌株对13种抗菌药物耐药,只有1株菌对所有抗菌药物敏感。采用PCR方法对羊源大肠杆菌分离株所携带的6种相关耐药基因进行检测,结果显示,6种耐药基因中的4种耐药基因blaTEM、proP-2、sul-Ⅰ、ampG检出率超过50%,耐药基因aph(3′)-Ⅰ携带率达40%,只有耐药基因aac(3)-Ⅱ检出率仅为5.5%。由此可见,内蒙古地区羊源大肠杆菌对13种抗菌药物产生了不同程度的耐药性,且存在严重的多重耐药情况,羊源大肠杆菌分离株携带aph(3′)-Ⅰ、sul-Ⅰ、ampG、blaTEM、proP-2、aac(3)-Ⅱ耐药基因。
In order to determine the resistant phenotype of sheep E. coli and the prevalence of its resistance genes in Inner Mongolia, microdilution assay was used to determine the minimum inhibitory concentrations (MICs) of 108 E.coli strains in Inner Mongolia to 13 commonly used antibiotics . The results showed that the resistance rates of the isolated strains to amoxicillin, cefalotin, sulfamethoxazole and colistin were the highest, reaching 100.0%, and the resistance rate to amoxicillin / clavulanic acid, tetracycline and ciprofloxacin Between 50% and 80%, the resistance rate to cefotaxime, meropenem and neomycin was less than 10%, which was more sensitive. 108 strains of E. coli resistant to more than seven kinds of drugs accounted for 94.4% of the strains, of which 15.6% strains of 13 kinds of antimicrobial resistance, only one strain of all antimicrobial susceptible. PCR-based method was used to detect six kinds of related resistance genes in sheep E.coli isolates. The results showed that four of the six resistance genes, blaTEM, proP-2, sul-Ⅰ and ampG, were detected The rate of aph (3) - Ⅰ was 40%, and only the detectable rate of aac (3) - Ⅱ was 5.5%. Thus, E. coli from Inner Mongolia had different degrees of resistance to 13 kinds of antibacterials, and there was a serious multi-drug resistance. The isolates of sheep-derived Escherichia coli carrying aph (3 ’) - Ⅰ, sul- Ⅰ, ampG, blaTEM, proP-2, aac (3) -Ⅱresistance genes.