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采用表面印迹法,以恩诺沙星为模板,甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,在聚苯乙烯酶标板表面直接合成恩诺沙星分子印迹聚合物膜。通过傅立叶红外光谱分析、电镜扫描、吸附平衡结合实验、Scatchard方程分析及吸附动力学实验对恩诺沙星印迹聚合物膜进行性能表征。合成的分子印迹聚合物膜具有很好的印迹效果,对恩诺沙星有较高的特异性吸附,且传质速率快,由Scatchard方程分析可知,聚合物膜中含有两类吸附位点,其中高亲和力位点的平衡解离常数(Kd)为19.49μg/mL,饱和吸附容量(Qmax)为12.98μg/mL,低亲和力位点的Kd为277.78μg/mL,Qmax为98.14μg/mL,吸附位点的异质性并不会影响聚合物膜应用于竞争性免疫吸附分析。通过该方法合成的恩诺沙星特异性识别聚合物膜可以作为仿生抗体,应用于竞争性免疫吸附分析检测恩诺沙星在食品中的残留。
Surface profiling was used to synthesize enrofloxacin as a template, methacrylic acid (MAA) as a functional monomer and ethylene glycol dimethacrylate (EDMA) as a cross-linker to synthesize directly on the polystyrene plate surface Enrofloxacin Imprinted Polymer Membrane. Fourier transform infrared spectroscopy, scanning electron microscopy, adsorption equilibrium binding experiments, Scatchard equation analysis and adsorption kinetics experiments were performed to characterize the enrofloxacin imprinted polymer films. The synthesized molecularly imprinted polymer membrane has a good imprinting effect, a higher specific adsorption on enrofloxacin, and a fast mass transfer rate. Scatchard equation analysis shows that the polymer membrane contains two types of adsorption sites, Among them, the equilibrium dissociation constant (Kd) of high affinity sites was 19.49 μg / mL, the saturation adsorption capacity (Qmax) was 12.98 μg / mL, the Kd of low affinity sites was 277.78 μg / mL, the Qmax was 98.14 μg / mL, Heterogeneity of adsorption sites does not affect the application of polymer membranes to competitive immunoabsorption assays. The enrofloxacin specific recognition polymer membrane synthesized by the method can be used as a biomimetic antibody and applied to competitive immunoadsorption analysis to detect enrofloxacin residue in food.