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目的:建立鹿茸药材中总磷脂含量测定方法,以评价不同商品地鹿茸药材的质量。方法:采用Folch试剂超声提取,钼蓝试剂显色,分光光度法测定。结果:样品中总磷脂含量以磷计的回归方程y=0.0205x+0.0032(r=0.9997),线性范围在2.3～80.5μg,平均回收率为93.01%,RSD=2.2%(n=5)。结论:该方法操作简便,方法学考察结果理想,可作为鹿茸药材质量控制的方法。
Objective: To establish a method for the determination of total phospholipids in pilose antler medicine to evaluate the quality of pilose antler medicine in different commercial products. Methods: Folch reagent ultrasonic extraction, molybdenum blue reagent color, spectrophotometric determination. Results: The regression equation of total phospholipid content in samples was y = 0.0205x + 0.0032 (r = 0.9997). The linear range was 2.3 ~ 80.5μg. The average recovery was 93.01% and the RSD was 2.2% (n = 5). Conclusion: The method is easy to operate and has ideal methodological results, which can be used as the quality control method of pilose antler.