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为了建立绣毛马铃苣苔的组培快繁技术,保护和开发利用绣毛马铃苣苔这一珍稀特有野生花卉资源,以MS作为基础培养基,研究植物激素和蔗糖对叶片不定芽诱导、增殖和生根的影响,筛选出绣毛马铃苣苔快速繁殖的最适培养基.结果表明:叶片表面、切口处均可直接诱导分化出不定芽,培养基MS+6-BA 0.5 mg/L+ KT 0.1 mg/L+NAA 0.3 mg/L +蔗糖25 g/L的诱导效果最好,诱导率达到100％,平均不定芽6.21个.培养基MS+16-BA 0.8 mg/L+NAA 0.2 mg/L+蔗糖30 g/L的不定芽增殖倍数最高,达到5.05倍;在不同浓度NAA的培养基中生根率均达到100％,适宜的浓度为NAA 0.8 mg/L,平均根数达到7.15条.该研究建立了绣毛马铃苣苔的离体再生技术,为其保护和利用奠定了基础.“,”The aim of this study was to establish the tissue culture and rapid propagation technology of O.dasyantha var. ferruginosa, so as to protect, develop and utilize this rare and endemic wild flower resources. MS was used as the basic medium to analyze the effect of plant hormones and sucrose on adventitious bud induction, proliferation and rooting of leaves, and the optimal medium for rapid propagation of O. dasyantha var. ferruginosa was screened out. The results showed that adventitious buds could be differentiated directly from leaf surface and incisions. The medium for adventitious buds wasMS+16-BA 0.5 mg/L+ KT 0.1 mg/L+NAA 0.3 mg/L+sugar 25 g/L with the bud induction rate of 100％, and the average number for each leaf was 6.21 buds. The medium of MS+ 16-BA 0.8 mg/L +NAA 0.2 mg/L +sugar 30 g/L had the highest multiplication of adventitious buds, with the proliferation of 5.05 times. For all treatments with different concentrations of NAA, the rooting ratio was 100％, the optimal concentrations for rooting was NAA 0.8 mg/L, and the average root number for plantlets was 7.15. The study established the regeneration technique in vitro of O. dasyantha var. ferruginosa and laid the foundation for protection and development of O. dasyantha var. ferruginosa.