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从江苏省稻瘟病不同优势小种菌株的液体培养液中提取稻瘟病菌粗毒素,以本地区较感病中粳稻新品系L119为材料,研究不同稀释浓度粗毒素对水稻种子萌发、成熟胚愈伤组织诱导及分化的影响;在诱导和分化培养基中分别加入适宜的稻瘟病菌粗毒素进行胁迫培养,以获得抗病性有所增强的变异植株。结果表明,种子萌发后胚芽的长度随着粗毒素浓度的提高而降低,在浓度达到1 m L/10 m L时种子萌发完全受到抑制。种胚愈伤组织的生长随着粗毒素浓度的增加受抑制程度逐渐增强,在浓度为25%时无愈伤产生;愈伤组织在分化时对粗毒素较敏感,在粗毒素浓度为5%时分化成苗数急剧下降。对诱导、分化2个阶段双重粗毒素胁迫培养的组培再生植株进行田间抗病性鉴定,抗病性较对照品种有所提高,说明利用稻瘟病菌粗毒素对水稻成熟胚组织的胁迫培养可以诱导愈伤组织的抗病性变异。
The crude toxin of Magnaporthe grisea was extracted from liquid culture broth of different dominant races of rice blast in Jiangsu Province. Using the new strain L119 of susceptible middle-season japonica rice in this region, the effects of different concentrations of crude toxin on germination and mature embryo of rice The effects of inducing and differentiation of injured tissues were studied. In the induction and differentiation medium, suitable crude extracts of Magnaporthe oryzae were added to the stress culture to obtain the mutants with enhanced disease resistance. The results showed that the germ length decreased with the increase of crude toxin concentration, and the seed germination was completely inhibited at the concentration of 1 m L / 10 m L. The growth of seed embryo callus increased gradually with the increase of crude toxin concentration and no callus was formed when the concentration was 25%. The callus was more sensitive to crude toxin during differentiation. At the crude toxin concentration of 5% When divided into a sharp decline in the number of seedlings. Induction and differentiation of two stages of crude toxin stress tissue culture of tissue culture regenerated plant disease resistance identification, disease resistance than the control varieties have increased, indicating that the use of crude extracts of Magnaporthe oryzae rice mature embryo tissue stress culture can Induction of callus variation in disease resistance.