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目的:研究不同浓度土三七水煎剂灌胃法诱导大鼠复制肝小静脉闭塞病(HVOD)动物模型。方法:土三七2 250 g水煎制成浓度为含生药1.5 g/ml的药液。80只雄性SD大鼠随机分为土三七高浓度组、中浓度组、低浓度组、正常组4组,每组20只,分别给予高浓度(15 g·kg-1·d-1)、中浓度(7.5 g·kg-1·d-1)、低浓度(3.75 g·kg-1·d-1)的土三七药液灌胃,正常组给予温开水灌胃。灌药1周后各组随机处死一半,剩余大鼠于给药2周后处死,采集血清和肝组织,检测血清学指标、观察肝组织病理学变化。结果:土三七高浓度组、中浓度组和低浓度组大鼠分别有78.95%(15/19)、68.42%(13/19)、57.89%(11/19)出现了HVOD病理表现;土三七高浓度组和中浓度组大鼠谷丙转氨酶(ALT)、总胆红素(TBIL)、总胆汁酸(TBA)均有不同程度显著升高(P<0.01);高浓度组较中浓度组作用更明显(P<0.01)。结论:土三七水煎剂灌胃法可以成功复制HVOD大鼠模型,其表现和病理改变与人类HVOD相似。
Objective: To study the effects of different concentrations of Sanqi Decoction (GVII) on gavage in rat model of hepatic venular occlusive disease (HVOD). Methods: soil thirty-two 2 250 g decoction made with a concentration of 1.5 g / ml crude drug solution. Eighty male Sprague-Dawley rats were randomly divided into three groups: high concentration (15 g · kg -1 · d -1), middle concentration group, low concentration group and normal group (n = 20) , Middle concentration (7.5 g · kg-1 · d-1) and low concentration (3.75 g · kg-1 · d-1) One week after irrigation, each group were randomly sacrificed to death, and the remaining rats were sacrificed two weeks after the administration. Serum and liver tissue were collected for detection of serological markers and pathological changes of liver. Results: The HVOD pathological changes were found in 78.95% (15/19), 68.42% (13/19) and 57.89% (11/19) groups respectively in the high, middle and low concentration groups. The levels of ALT, TBIL and TBA in Panax notoginseng high and middle concentration groups all increased to different extents (P <0.01) Concentration group more obvious (P <0.01). Conclusion: Rhizoma Imperatae decoction gavage can successfully replicate the HVOD rat model, its performance and pathological changes are similar to human HVOD.