论文部分内容阅读
目的研究羊水中基质金属蛋白酶(MMP)-8和白细胞介素(IL)-6在早产临产患者中的发生率及其意义。方法选择于2010年1月-2012年12月分娩的单胎初产妇200例作为研究对象,按分娩时孕周分为早产临产组(n=102)和足月临产组(n=98)。用酶联免疫吸附试验分别检测孕妇羊水和血清中MMP-8及IL-6的表达,同时行羊水细菌培养。结果早产临产组与足月临产组孕妇羊水中MMP-8水平分别为(320.45±59.88)、(153.72±29.12)ng/m L,差异有统计学意义(P<0.05);但早产临产组与足月临产组孕妇血清MMP-8水平分别为(9.56±2.11)、(9.42±2.01)ng/m L,差异无统计学意义(P>0.05)。早产临产组与足月临产组孕妇羊水IL-6水平分别为(90.5±16.3)、(20.6±12.5)μg/L,血清IL-6水平分别为(159.2±20.4)、(22.3±11.8)μg/L,组间差异均有统计学意义(P<0.05)。早产临产组与足月临产组孕妇羊水中致病菌培养阳性率分别为8.8%(9/102)、1.0%(1/98),差异有统计学意义(P<0.05)。结论宫内感染在早孕临产组孕妇中比例较高,是导致早产的主要因素;羊水MMP-8水平的升高、羊水及血清中IL-6水平的升高对预测早产有一定临床应用价值,尤其是血清IL-6水平升高可以作为一项临床应用开展。
Objective To study the incidence and significance of matrix metalloproteinase (MMP-8) and interleukin (IL) -6 in amniotic fluid in preterm labor. Methods A total of 200 singleton primiparous women who gave birth between January 2010 and December 2012 were selected as the study group and divided into preterm delivery group (n = 102) and full term delivery group (n = 98) according to gestational age at delivery. Enzyme-linked immunosorbent assay were used to detect the expression of MMP-8 and IL-6 in amniotic fluid and serum of pregnant women respectively. At the same time, amniotic fluid bacterial culture was performed. Results The levels of MMP-8 in amniotic fluid of preterm delivery group and term delivery group were (320.45 ± 59.88) and (153.72 ± 29.12) ng / m L, respectively, with statistical significance (P <0.05) The serum levels of MMP-8 in pregnant term labor group were (9.56 ± 2.11) and (9.42 ± 2.01) ng / m L, respectively, with no significant difference (P> 0.05). The level of IL-6 in amniotic fluid in preterm delivery group and term delivery group were (90.5 ± 16.3) and (20.6 ± 12.5) μg / L respectively, and the levels of serum IL-6 were (159.2 ± 20.4) and (22.3 ± 11.8) / L, the differences between the groups were statistically significant (P <0.05). The positive rates of pathogenic bacteria culture in amniotic fluid of pregnant women in preterm delivery group and term delivery group were 8.8% (9/102) and 1.0% (1/98), respectively. The difference was statistically significant (P <0.05). Conclusions The intrauterine infection is a major factor leading to preterm birth in the first trimester of pregnancy. The elevated level of MMP-8 in amniotic fluid and the elevated level of IL-6 in amniotic fluid and serum have some clinical value in predicting preterm birth. In particular, elevated serum IL-6 level can be used as a clinical application.