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目的寻求一种适宜的石棉表面改性剂。方法利用斑点杂交和流式细胞技术观察柠檬酸铝、混合稀土或亚硒酸钠等３种化合物对温石棉致人胚肺（ＨＥＬ）细胞转化过程中ｃＨａｒａｓ癌基因和Ｐａｎｒａｓ小鼠抗体（Ｐ２１ｒａｓ）蛋白的影响。结果在温石棉染毒组，ｃＨａｒａｓ癌基因的转录水平及Ｐ２１ｒａｓ蛋白含量明显高于对照组。在上述３种化合物预处理的温石棉组，ＨＥＬ细胞ｃＨａｒａｓ癌基因的转录水平及Ｐ２１ｒａｓ蛋白含量均明显低于未处理温石棉组。结论用上述３种化合物预处理温石棉，有可能减轻温石棉对人类的致癌危害性。
Objective To find a suitable asbestos surface modifier. Methods Hybridization and flow cytometry were used to observe the effects of aluminum citrate, mixed rare earths or sodium selenite on cHaras oncogene and Panras mice during the transformation of human embryonic lung (HEL) cells induced by chrysotile Antibody (P21ras) Protein Impact. Results in chrysotile exposure group, c Hassa oncogene transcription and P21ras protein content was significantly higher than the control group. In chrysotile treated with the above three compounds, the transcriptional level of c-Ha-Ras oncogene and the content of P21ras in HEL cells were significantly lower than those in untreated chrysotile. Conclusion Pretreatment of chrysotile with these three compounds may reduce the carcinogenicity of chrysotile to humans.