论文部分内容阅读
分别以硅烷化磁铁粉、硅烷磁化脱乙酰几丁质及脱乙酰几丁质作载体，甲醛或戊二醛为交联剂，对胰蛋白酶的固定化条件及所制备的固定化酶的性质进行了研究．研究了载体种类、交联剂和ｐＨ值以及载体与酶比例等因素对胰蛋白酶固定化的影响．研究了不同载体固定胰蛋白酶的特点，证明以硅烷化试剂和甲醛作交联剂的硅烷化磁铁粉作胰蛋白酶固定化的载体具有明显优点，所制备的硅烷化磁铁粉固定化胰蛋白酶酶学性质明显优于可溶性胰蛋白酶
Respectively, silanized magnet powder, silane chitosan and chitosan as a carrier, formaldehyde or glutaraldehyde as cross-linking agent, the immobilization conditions of trypsin and the properties of the immobilized enzyme prepared Study. The effects of carrier type, cross-linking agent and pH value, and the ratio of carrier to enzyme on the immobilization of trypsin were studied. The characteristics of immobilized trypsin with different carriers were studied. It is proved that silylated magnet powder with silanization reagent and formaldehyde as the carrier for trypsin immobilization has obvious advantages. The immobilized trypticase immobilized trypticase Nature is better than soluble trypsin