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介绍了水热合成CdSe半导体纳米晶的工艺,调节反应温度及时间有助于加速CdSe纳米晶的生长,使其荧光产率得到显著提高;在一定的初始条件下,随着纳米晶核长大,产物的荧光产率单调增至极值然后逐渐下降;CdSe荧光产率的最大值与反应温度关系密切。实验利用1-乙基–1-3-(3-二甲基氨基-丙基)–碳化二亚胺将所制备的CdSe与转铁蛋白偶联成生物探针,成功标记了人肺腺癌细胞。
The process of hydrothermally synthesizing CdSe semiconductor nanocrystals was introduced. The reaction temperature and time were adjusted to accelerate the growth of CdSe nanocrystals. The yield of CdSe nanocrystals was significantly improved. Under certain initial conditions, with the growth of nanocrystals, , The fluorescence yield of the product monotonically increased to the extreme value and then decreased gradually. The maximum fluorescence yield of CdSe was closely related to the reaction temperature. Experiments were carried out using 1-ethyl-1-3- (3-dimethylamino-propyl) -carbodiimide to conjugate the prepared CdSe with transferrin into bio-probes and successfully labeled human lung adenocarcinoma cell.