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目的进一步研究苯酐哮喘的发病机制，建立苯酐哮喘大鼠模型并观察其体内抗原特异性ＩｇＧ和ＩｇＥ的变化。方法苯酐致敏大鼠抗原攻击后描记呼吸曲线以监测气道反应，酶联免疫吸附试验及被动皮肤致敏试验检测抗原特异性ＩｇＧ和ＩｇＥ。结果苯酐可引起大鼠气道高反应性和体内抗原特异性ＩｇＧ和ＩｇＥ水平升高。结论苯酐哮喘属半抗原致敏变应性哮喘。该实验所建模型可用于苯酐哮喘发病机制的进一步研究
OBJECTIVE To further study the pathogenesis of phthalic anhydride in asthmatic rats and to establish a rat model of phthalic anhydride and asthma and to observe the changes of antigen-specific IgG and IgE in vivo. Methods Antigen-specific IgG and IgE were detected after traumatic sensitization with phthalic anhydride in rat respiratory tract. The airway response, enzyme-linked immunosorbent assay and passive skin sensitization were used to detect the antigen-specific IgG and IgE. Results Phthalic anhydride induced airway hyperresponsiveness and elevated levels of antigen-specific IgG and IgE in vivo. Conclusion Phthalocyanine is a hapten-sensitized allergic asthma. The experimental model can be used for the further study of the pathogenesis of phthalic anhydride in asthma