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在过氧化物酶一抗过氧化物酶(PAP)的免疫组化的染色中,常常遇到第一抗体价格昂贵,难以获得的问题。作者介绍了一种节约抗血清的方法。用胶片切割成一个方框,外径2.5×2.5cm(内径2.0×2.0cm)。在方框的上下表面均匀地抹上一层凡士林,把胶片框放在载玻片上组织切片的周围。用微量吸管将抗血清滴到切片上(约20μ1/cm~2)。将第二张组织切片的载玻片面朝下,与第一张载玻片成一定的角度
In the immunohistochemical staining of peroxidase primary antibody peroxidase (PAP), the first antibody is often encountered with a problem that is expensive and difficult to obtain. The author introduced a method to save antisera. The film was cut into a square with an outer diameter of 2.5 x 2.5 cm (inner diameter 2.0 x 2.0 cm). Apply a layer of petroleum jelly evenly to the upper and lower surfaces of the box and place the film frame around the tissue section on a glass slide. The antiserum was dropped onto the sections with a micropipette (approximately 20 μl / cm 2). Slide the slide of the second tissue slice face down and at an angle to the first slide