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目的比较单染和复染染色方法制作旋毛虫肌幼虫囊包标本的效果。方法单染,醋酸明矾卡红染色液染色:①染色24h后,分色;②染色24h,不分色。复染,醋酸明矾卡红染色液与固绿染色液染色:③醋酸明矾卡红染色24h后,分色;固绿染色20s;④醋酸明矾卡红染色24h,不分色;固绿染色20s。结果方法①制作的标本囊包轮廓清晰,囊内幼虫体态明显;方法③制作标本,囊包与周围肌细胞分界明显,立体感强;囊内幼虫、囊壁内层、囊壁外层相互之间层次分明,特征典型。结论方法①和③制作的标本,染色效果好,易于观察。
Objective To compare the effect of single-stained and double-stained dyeing on the paraffin-embedded samples of muscle larvae of Trichinella spiralis. Methods Single stain, alum card red acetate staining: ① staining 24h, separation; ② staining 24h, no color. Stained with alum acetate red stained solution and solid green stained solution. ③After stained with acetic alum card red for 24 hours, the color was separated; solid green stained for 20 seconds; ④ acetate alum red stained for 24h, no color separation; solid green stained for 20 seconds. Results ① The outline of the capsule produced by the method was clear, and the larvae in the larvae had a clear body. Methods ③ The specimens were made. The demarcation between the capsule and the surrounding muscle cells was obvious and the three-dimensional sensation was strong. The larvae, the inner layer of the capsule and the outer layer of the capsule Between layers, typical characteristics. Conclusion Methods ① and ③ produced specimens, staining effect is good, easy to observe.