高灵敏脱氧核糖核酸(DNA)扩增技术聚合酶链反应(PCR)的出现,提出了由孕母外周血分离胎儿细胞用于产前诊断的可能。有关研究集中在富集或分离母血中的胎儿有核红细胞或滋养细胞。使用有核红细胞由荧光原位杂交(FISH)技术鉴定胎儿染色三体和检出单体已均有报道。妊娠5～6周母血中可能有来自于胎儿的滋养细胞。针对不同滋养细胞抗原的磁免疫和荧光免疫技术均已被用来富集母血中的滋养细胞。本文使用两种不同的磁免疫方法,由识别滋养细胞叶酸结合蛋白的单克隆抗体LK26建立模型系统,正向富 The emergence of polymerase chain reaction (PCR), a highly sensitive DNA amplification technique, has proposed the possibility of prenatal diagnosis of fetal cells isolated from peripheral blood of pregnant mothers. Research has focused on fetal erythroblasts or trophoblasts that enrich or isolate maternal blood. The use of nucleated erythrocytes by fluorescence in situ hybridization (FISH) to identify fetal trisomy and monomer detection have been reported. 5 to 6 weeks of pregnancy in maternal blood may have from the fetus of trophoblast cells. Magnetic immunofluorescence and immunofluorescence techniques targeting different trophoblastic antigens have been used to enrich trophoblast cells in maternal blood. This article uses two different methods of magnetic immunization, the recognition of trophoblast folate-binding protein monoclonal antibody LK26 model system, forward rich