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目的探讨膜雌激素持续作用对乳腺癌细胞生长调控的影响及其可能的分子机制。方法采用MTT法测不同浓度的膜雌激素对乳腺癌细胞MCF-7生长的影响。分别采用MTT法和RT-PCR法分析不同的信号抑制剂调控后,膜雌激素对乳腺癌细胞MCF-7生长及其EGFR mRNA表达水平的影响。结果终浓度为10-7M和10-12M的膜雌激素持续作用能诱导乳腺癌MCF-7细胞的增殖。膜G蛋白藕联受体阻断剂苏拉明和EGFR阻断剂AG-1478能明显抑制膜雌激素对MCF-7细胞生长的诱导作用。苏拉明和AG-1478能抑制膜雌激素持续作用上调MCF-7细胞中EGFRmRNA表达的作用。结论雌激素膜持续效应能刺激乳腺癌细胞的增殖,这一作用是通过膜G蛋白藕联受体和EGFR通路来完成的。
Objective To investigate the effect of membrane estrogen on the growth of breast cancer cells and its possible molecular mechanisms. Methods The effects of different concentrations of estrogen on the growth of MCF-7 breast cancer cells were determined by MTT assay. The effect of membrane estrogen on the growth of MCF-7 breast cancer cells and the expression of EGFR mRNA were analyzed by MTT assay and RT-PCR assay respectively. Results The persistence of membrane estrogen at final concentrations of 10-7 M and 10-12 M induces the proliferation of breast cancer MCF-7 cells. Supramide, an inhibitor of membrane G protein-coupled receptor, and AG-1478, an EGFR blocker, significantly inhibited the induction of membrane estrogen on the growth of MCF-7 cells. Suramin and AG-1478 inhibit the sustained effect of membrane estrogen on the up-regulation of EGFR mRNA expression in MCF-7 cells. Conclusion The sustained effect of estrogen membrane can stimulate the proliferation of breast cancer cells through the membrane G protein-coupled receptor and EGFR pathway.