论文部分内容阅读
目的探讨肿瘤坏死因子(TNF)a在胎膜早破发病机制中的作用及意义。方法采集无任何妊娠并发症、临产前要求剖宫产分娩的初产妇(包括35例足月胎膜早破、15例足月前胎膜早破和作对照的17例正常未破膜者)血、羊水及脐带血,用双抗体夹心酶联免疫吸附实验法胎膜常规苏木素伊红染色后行组织病理学检查。结果①胎膜早破组血清、羊水、脐带血中TNFa浓度明显高于对照组,差异有显著性(P<0.05或<0.01):但足月和足月前胎膜早破两组间血清、羊水中TNF浓度、胎膜感染率均无显著性(P>0.05)。②胎膜有感染组血清、羊水脐带血中TNFa浓度明显高于无感染组,差异有显著性(P<0.01);在无感染组中胎膜早破门血清、羊水中,FNFa浓度,也较对照组高,差异有显著性(P<0.01)。③所有研究对象血清、羊水、脐带血中TNFa浓度呈明显正相关(r=0.386,P<0.01)。结论TNFa在胎膜早破发病机制中有促炎、降解。
Objective To investigate the role and significance of tumor necrosis factor (TNF) a in the pathogenesis of premature rupture of membranes. Methods Collecting primipara (including 35 cases of full-term premature rupture of membranes, 15 cases of full-term premature rupture of membranes and control of 17 cases of normal unruptured) without any complications of pregnancy, prenatal cesarean delivery, Blood, amniotic fluid and umbilical cord blood were collected and histopathologically examined with conventional hematoxylin and eosin staining using double antibody sandwich enzyme-linked immunosorbent assay. Results ① The concentration of TNFa in serum, amniotic fluid and umbilical cord blood in premature rupture of membranes was significantly higher than that in control group (P <0.05 or <0.01). However, the serum levels of TNFa in preterm premature rupture of membranes , The concentration of amniotic fluid TNF, fetal infection rate was not significant (P> 0.05). (2) The concentration of TNFa in the serum of cord blood with amniotic fluid and cord blood of amniotic fluid was significantly higher than that of non-infected group (P <0.01). In the non-infected group, the concentration of FNFa in premature rupture of membranes, amniotic fluid and amniotic fluid The control group, the difference was significant (P <0.01). ③ The concentrations of TNFa in serum, amniotic fluid and umbilical cord blood of all the subjects were positively correlated (r = 0.386, P <0.01). Conclusions TNFa is proinflammatory and biodegradable in the pathogenesis of premature rupture of membranes.