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目的 寻找皖南尖吻蝮蛇毒中的抗癌组分并初步研究其性质。方法 以DEAE SepharoseFF ,SOURCE 30S柱层析纯化粗毒 ;以MTT法检测细胞毒活性 ;以SDS PAGE(银染 )测定纯度 ;以还原性及非还原SDS PAGE测定分子量 ;又测定了它们的热稳定性和pH稳定性。结果 得到两个电泳纯的蛇毒类抗癌组分 ,分别命名为ACTX 6和ACTX 8,分子量为 98kDa和 2 7kDa ,都不是糖蛋白 ,其中ACTX 6由两个分子量相近的亚单位以二硫键连接而成 ;它们对肺癌细胞A5 4 9最敏感 ;ACTX 6对热稳定而ACTX 8对热不稳定 ;ACTX 6的稳定pH范围为 7- 9,而ACTX 8在 6 - 9的范围内活性都比较稳定
Aim To find anticancer components in Agkistrodon acutus venom and study its properties. Methods The crude drug was purified by DEAE Sepharose FF and SOURCE 30S column. The cytotoxic activity was determined by MTT assay. The purity was determined by SDS PAGE (silver staining). The molecular weights were determined by reducing and non-reducing SDS PAGE. The thermal stability Sexual and pH stability. As a result, two electrophoresis pure snake venom anticancer components were obtained, named as ACTX 6 and ACTX 8, with molecular weights of 98 kDa and 27 kDa, respectively, which were not glycoproteins. ACTX 6 consisted of two subunits of similar molecular weight with disulfide bonds ; They are most sensitive to lung cancer cells A549; ACTX6 is thermally stable but ACTX8 is thermally unstable; ACTX6 has a stable pH range of 7- 9; ACTX 8 is active in the range of 6-9 relatively stable