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经不同间隔天数及针次接种肾综合征出血热沙鼠肾细胞灭活疫苗（１：０、７、２８天，Ⅱ：０、１４、２８天，Ⅲ：０、２８天，Ⅳ：０、２８、１８０天，Ⅴ：０时接种２针，Ⅵ：方案Ⅴ基础上，第３０天再种１针），１年以后对方案Ⅰ、Ⅱ、Ⅳ、Ⅵ加强１针。于基础免疫末针２周后及加强前后采静脉血，用ＨＩ、ＩＦＡ、ＲＰＨＩ３种方法检测特异性抗体，对其阳转率和几何均数进行分析。结果表明，方案Ⅰ与方案Ⅱ检测结果无显著性差别，提示方案Ⅱ可以替代方案Ⅰ。方案Ⅲ优于方案Ⅴ，说明在剂量相同的情况下，分两次较１次接种效果好。基础免疫１年后，多数人抗体已转阴，因此，对加强免疫时间应进一步研究。
The mice were inoculated intraperitoneally with different doses of Hemorrhagic Fever with Renal Syndrome (1: 0,7,28 days, Ⅱ: 0,14,28 days, Ⅲ: 0,28 days, Ⅳ: 0, 28,180 days, 2 doses of Ⅴ: 0, Ⅵ: 1 dose of the other doses on the 30th day). After 1 year, the needles of 1, 2, 4 and 6 were strengthened. Two weeks after the basal immunization, venous blood was harvested before and after intensive care. The specific antibodies were detected by HI, IFA, and RPHI, and their positive rate and geometric mean were analyzed. The results showed that there was no significant difference between the results of scheme Ⅰ and scheme Ⅱ, suggesting that scheme Ⅱ could replace scheme Ⅰ. Scenario III is superior to Scenario V, indicating that in the case of the same dose, the effect of inoculation twice in two times is better. After 1 year of basic immunization, most of the human antibodies have become negative, therefore, further studies should be conducted on the time of booster immunization.