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对人群的麻疹抗体水平监测,目前都采用血凝抑制法,此法虽简便快速,但许多地区因购买猴血球困难无法开展此项工作。本文通过对麻苗免疫前后和健康人群麻疹抗体的测定,比较酶联吸附法(ELISA)和血凝抑制法(HI)敏感性和特异性,结果表明ELISA法可以代替HI法进行麻疹的抗体水平监测。 1 材料与方法法 1.1 ELISA-IgG法 ELISA间接法,以OD值≥2.1的血清最高稀释度为其效价。麻疹人群抗体水平测定血清稀释度<1:200为阴
Measles antibody levels in the crowd monitoring, are using hemagglutination inhibition method, although this method is quick and easy, but many areas due to difficulties in the purchase of monkey blood cells can not carry out this work. In this paper, the detection of measles antibody before and after the immune response to Miao Miao, enzyme-linked immunosorbent assay (ELISA) and hemagglutination inhibition (HI) sensitivity and specificity, the results show that the ELISA method can be used in place of the HI method for measles antibody levels monitor. 1 Materials and Methods 1.1 ELISA ELISA ELISA indirect ELISA method to OD value ≥ 2.1 serum highest dilution of its potency. Measles population antibody levels measured serum dilution <1: 200 for the shade