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用30Gy、90Gy和180Gy 12C6+重离子辐照油菜干种子,研究其对油菜M1代的诱变效应。结果表明不同剂量12C6+重离子辐照使油菜的出苗率、株高和开花率有不同程度的提高,并使开花期提早;30Gy辐照使单株角果数和单株产量有了一定程度的提高;三种辐照剂量都造成了花粉生活力、千粒重和含油量的降低。RAPD扩增结果表明,42个随机引物中有13个引物扩增出差异条带,30Gy、90Gy和180Gy引起的RAPD变异率分别为22.1%、23.7%和36.2%。研究表明,12C6+重离子辐射能有效地引起油菜DNA序列发生改变,从而诱导基因变异,为油菜育种提供丰富的种质材料。
The dry seeds of rapeseed were irradiated with 30Gy, 90Gy and 180Gy 12C6 + heavy ions to study their mutagenic effects on the M1 generation of rapeseed. The results showed that different doses of 12C6 + heavy ion irradiation rape seedling emergence rate, plant height and flowering rate increased to some extent, and the flowering period earlier; 30Gy irradiation so that the number of single plantlets and per plant yield to a certain extent Increase; three doses of radiation have caused pollen viability, grain weight and oil content decreased. RAPD amplification results showed that 13 out of 42 random primers amplified differential bands, and the mutation rates of RAPD caused by 30Gy, 90Gy and 180Gy were 22.1%, 23.7% and 36.2%, respectively. Studies have shown that 12C6 + heavy ion radiation can effectively change the DNA sequence of rapeseed so as to induce genetic variation and provide abundant germplasm materials for rapeseed breeding.