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目的 :探讨大鼠钙超载心肌顿抑时心脏收缩功能的变化及粉防己碱 (Tet)的保护作用。方法 :将 46只成年 SD大鼠随机分成对照、心肌缺血、心肌顿抑、大剂量和小剂量 Tet组 ,另 10只大鼠用于确定钙超载。给大鼠注射维生素 D3(Vit D3,30万 U/ kg)和烟酸 ,16 d后检测心肌细胞 Ca2 +浓度 ([Ca2 + ]i)。确认达到钙超载标准后 ,结扎左前降支 ,至 2 0 min心肌缺血 +6 0min再灌注 ,动态监测 dp/ dtmax和 Vmax,实验结束后测定各组心肌 [Ca2 + ]i。 Tet组在注射 Vit D3的同时及以后每日分别给予Tet6 2 .2和 93.6 μmol/ kg,(灌胃 ) ,16 d后 ,致心肌缺血和再灌注 ,测定心肌收缩功能和 [Ca2 + ]i。结果 :给 Vit D3后第 16天 ,[Ca2 + ]i较对照水平增加 2 .6倍 [(146 .8± 10 .8)对 (36 8.5± 2 2 .6 ) nm ol/ L ,(P <0 .0 1) ];Tet组 ,分别为 (2 10 .8± 16 .4)和(198.6± 15 .3) nmol/ L ,明显低于钙超载组 (P<0 .0 1)。缺血前钙超载组心脏收缩功能明显低于对照组 (dp/ dtmax和 Vmax分别为对照组的 76 .6 %和 6 8.4% )。 2 0 m in心肌缺血致各组 dp/ dtmax和 Vmax均明显下降 (P<0 .0 1) ,以顿抑组和钙超载组最为明显。再灌注后心肌收缩功能逐步恢复。在 Tet组 ,各时间点 dp / dtmax和 Vmax均高于顿抑组和钙超载组 ,大剂
Objective: To investigate the changes of cardiac contractile function and the protective effect of tetrandrine (Tet) during myocardial stunning in rats. Methods: Forty-six adult SD rats were randomly divided into control, myocardial ischemia, myocardial stunning, high-dose and low-dose Tet groups, and the other 10 rats were used to determine calcium overload. Rats were injected with vitamin D3 (3.0 million U / kg) and nicotinic acid, and the cardiomyocyte Ca2 + concentration ([Ca2 +] i) was measured 16 days later. After reaching the calcium overload standard, the left anterior descending coronary artery was ligated and reperfusion was performed at 60 min after myocardial ischemia for 60 min. The dp / dtmax and Vmax were monitored dynamically. After the experiment, myocardial [Ca2 +] i was measured. The Tet group was given Tet6 2 .2 and 93.6 μmol / kg daily at the same time and after Vit D3 injection respectively. After 16 days, the myocardial ischemia and reperfusion were induced in Tet group. The myocardial contractile function and [Ca2 +] i. RESULTS: On the 16th day after Vit D3 administration, [Ca2 +] i increased by 2.6 times [(146.8 ± 10.8) vs (36 8.5 ± 2.2 2.6) nm ol / L, (P <0.01)]; Tet group were (2 10.8 ± 16.4) and (198.6 ± 15.3) nmol / L, respectively, which was significantly lower than that of calcium overload group (P <0.01). Cardiac contractile function in pre-ischemic calcium overload group was significantly lower than that in control group (d6 / dtmax and Vmax were 76.6% and 6 8.4%, respectively) in the control group. The dp / dtmax and Vmax of 2 0 m in myocardial ischemia group were significantly decreased (P <0.01), the most obvious was the group of Dayton suppression and calcium overload. Myocardial contractile function gradually restored after reperfusion. In Tet group, dp / dtmax and Vmax at each time point were significantly higher than those in the control group and calcium overload group