本文研究了cAMP,cGMP及cAMP磷酸酯对艾氏腹水癌细胞中DNA聚合酶α活性的影响。实验结果说明这些化合物对无细胞体系中DNA聚合酶α无作用。反之浓度为5×10~(-7)M/ml的cAMP、cAMP-丁酯及cAMP-苄酯能抑制完整腹水癌细胞中DNA聚合酶α的活性,而cAMP-环己酰胺则无此作用,说明这些化合物是通过抑制DNA前身参入DNA而不是直接抑制DNA聚合酶催化的聚合反应。并且此抑制作用与化合物的结构有关。 This study investigated the effects of cAMP, cGMP and cAMP phosphates on DNA polymerase alpha activity in Ehrlich ascites carcinoma cells. The experimental results show that these compounds have no effect on the DNA polymerase alpha in the cell-free system. In contrast, cAMP, cAMP-butyl ester, and cAMP-benzyl ester at a concentration of 5×10 -7 M/ml can inhibit the activity of DNA polymerase α in intact ascites cancer cells, whereas cAMP-cyclohexanamide has no such effect. This indicates that these compounds inhibit DNA polymerase-catalyzed polymerization by inhibiting DNA precursors from entering DNA rather than directly inhibiting it. And this inhibition is related to the structure of the compound.