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目的了解白介素4(IL-4)在白念珠菌系统性感染中所起的作用。方法建立健康小鼠及地塞米松诱导的免疫抑制小鼠系统性白念珠菌感染模型,用酶联免疫吸附试验(ELISA)及逆转录-聚合酶链反应分别检测脾内IL-4及mRNA水平,用平皿稀释法检测肾内菌落形成单位(CFU),并记录平均生存时间。结果健康鼠致死量感染后3d、7d脾中IL-4蛋白水平分别为(124.8±24.1)pg/g、(262.8±21.8)pg/g(P<0.001),肾内带菌量分别为(21.25±6.31)×102CFU、(57.52±10.41)×102CFU(P<0.01);免疫抑制鼠组感染后3d、7d脾中IL-4蛋白水平分别为(85.3±18.8)pg/g、(140.7±37.2)pg/g(P<0.05),肾内带菌量分别为(12.00±2.80)×102CFU、(68.75±13.81)×102CFU(P<0.001)。上述两组感染后3d、7d脾中mRNA水平表达趋势与其IL-4蛋白水平相似。致死量组平均生存时间16d,免疫抑制鼠组平均生存时间12.4d。结论IL-4可能参与实验性小鼠白念珠菌系统性感染的发病。
Objective To understand the role of interleukin-4 (IL-4) in the systemic infection of Candida albicans. Methods The model of systemic Candida albicans infection was established in healthy mice and dexamethasone-induced immunosuppressed mice. The levels of IL-4 and mRNA in the spleen were detected by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction The platelet dilution method was used to detect colony forming units (CFU) in the kidney and the mean survival time was recorded. Results The levels of IL-4 protein in the spleens of mice on the 3rd and 7th day after infection were (124.8 ± 24.1) pg / g and (262.8 ± 21.8) pg / g respectively (P <0.001) (85.3 ± 18.8) pg / g, (140.7 ± 37.2 ± 3.81) × 102CFU, (57.52 ± 10.41) × 102CFU, respectively (P <0.01) ) pg / g (P <0.05). The intrarenal inoculation rates were (12.00 ± 2.80) × 102CFU and (68.75 ± 13.81) × 102CFU respectively (P <0.001). The expression level of spleen mRNA in the two groups after infection on the 3rd and 7th day was similar to that of the IL-4 protein. The average lethal time of the lethal dose group was 16 days and that of the immunosuppressed group was 12.4 days. Conclusion IL-4 may be involved in the pathogenesis of systemic Candida albicans infection in mice.