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目的：研究神经生长因子（ＮＧＦ）对缺血性脑损伤中神经细胞凋亡的影响。方法：采用大鼠全脑缺血模型，ＴＵＮＥＬ方法原位标记ＤＮＡ片段，观察脑室内注射ＮＧＦ后海马ＣＡ１区神经细胞凋亡的变化。结果：使用ＮＧＦ后３日海马ＣＡ１区ＴＵＮＥＬ阳性神经细胞数为锥体细胞总数的１３．９％±１１．６％，与缺血对照组（２１．３％±１３．７％）比较显著减少（Ｐ＜０．０１）。结论：外源性ＮＧＦ对脑缺血所致的神经细胞凋亡可能具有一定的保护作用
Objective: To investigate the effect of nerve growth factor (NGF) on the apoptosis of nerve cells in ischemic brain injury. Methods: DNA fragments were labeled in situ by rat brain ischemia model and TUNEL method. Apoptosis of hippocampal CA1 neurons was observed after intracerebroventricular injection of NGF. Results: The number of TUNEL-positive neurons in hippocampal CA1 region was 13.9% ± 11.6% of the total number of pyramidal cells in the hippocampus on the 3rd day after NGF administration, significantly decreased compared with the ischemic control group (21.3% ± 13.7%) (P <0.01). CONCLUSION: Exogenous NGF may have some protective effects on the apoptosis of neural cells induced by cerebral ischemia