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目的观察三氧化二砷(ATO)对肝癌细胞系Hep G2和Huh-7的影响,并探讨其作用机制。方法体外培育肝癌细胞Hep G2和Huh-7,选取不同浓度(0、2、4μmol/L)的ATO进行孵育细胞,倒置显微镜观察细胞形态学改变,四甲基偶氮唑蓝(MTT)法检测ATO对两种不同肝癌细胞增殖情况的影响,流式细胞术观察细胞凋亡情况,蛋白印迹法检测细胞中Bcl-2111(Bim)的表达。结果不同浓度(0、2、4μmol/L)的ATO对两种细胞系的生长起到不同程度的抑制,且呈浓度依赖性,受抑制情况两组差异无统计学意义。ATO对两组细胞系的促凋亡作用明显,两组细胞在ATO的作用下Bim均呈高表达。结论ATO对肝癌细胞Hep G2和Huh-7具有诱导凋亡的作用。
Objective To observe the effect of arsenic trioxide (ATO) on Hep G2 and Huh-7 cell lines and to explore its mechanism. Methods Hep G2 and Huh-7 cells were cultured in vitro. The cells were incubated with different concentrations of ATO (0, 2, 4 μmol / L). The morphological changes were observed with inverted microscope. MTT assay ATO on the proliferation of two different types of hepatocellular carcinoma cells were observed by flow cytometry. Western blotting was used to detect the expression of Bcl-2111 (Bim). Results ATO at different concentrations (0, 2, 4μmol / L) inhibited the growth of the two cell lines in different degrees, and there was no significant difference between the two groups in a concentration-dependent manner. ATO on the two groups of cell apoptosis effect was obvious, two groups of cells under the action of ATO Bim were highly expressed. Conclusion ATO can induce apoptosis of Hep G2 and Huh-7 cells.