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目的研究幽门螺杆菌L型(Helicobacter pyloriL-form,H.pylori-L型)感染,Ras相关区域家族1A基因(RASSF1A)和细胞S期激酶相关蛋白2(Skp2)在胃癌发生、发展中的作用及相互关系。方法应用革兰染色和免疫组织化学SP法检测50例胃癌组织及20例癌旁正常组织中的H.pylori-L型感染情况;同时应用逆转录聚合酶链式反应(RT-PCR)和免疫组织化学SP法检测上述组织中癌基因skp2和抑癌基因RASSF1A的表达。结果 50例胃癌组织标本中免疫组化及革兰染色H.pylori-L型同时阳性的病例有30例,胃癌组织和癌旁正常组织中H.pylo-ri-L型阳性率分别为60.0%和20.0%,2组之间差异有统计学意义(P<0.05)。胃癌组织中Skp2表达阳性率明显高于对照组(P<0.01);而RASSF1A表达阳性率明显低于对照组(P<0.01);H.pylori-L型阳性组的Skp2表达阳性率高于H.pylori-L型阴性组(P<0.05);H.pylori-L型阳性与Skp2的表达阳性呈正相关关系;RASSF1A的表达与H.pylori-L型阳性呈负相关关系。结论 H.pylori-L型感染在胃癌的发生发展过程中起重要的作用,其促进胃癌发生、发展的机制可能涉及上调Skp2的表达和下调RASSF1A的表达。
Objective To study the role of Helicobacter pylori L-form (H.pylori-L) infection, RASSF1A and Skp2 in the development and progression of gastric cancer And interrelationships. Methods Gram stain and immunohistochemical SP method were used to detect H.pylori-L infection in 50 gastric cancer tissues and 20 adjacent normal tissues. RT-PCR and immunofluorescence The histochemical SP method was used to detect the expression of oncogene skp2 and tumor suppressor gene RASSF1A in the above tissues. Results The positive rate of H.pylori-L type in 50 cases of gastric cancer specimens was 30 cases, and the positive rates of H.pylo-ri-L in gastric cancer tissues and adjacent normal tissues were 60.0% And 20.0% respectively. There was a significant difference between the two groups (P <0.05). The positive rate of Skp2 in gastric cancer tissues was significantly higher than that in control group (P <0.01), while the positive rate of RASSF1A expression was significantly lower than that in control group (P <0.01). The positive rate of Skp2 in H.pylori-L positive group was higher than that in H (P <0.05). The positive expression of H.pylori-L was positively correlated with the expression of Skp2. The positive expression of RASSF1A was negatively correlated with the positive expression of H.pylori-L. Conclusion H.pylori-L infection plays an important role in the carcinogenesis and progression of gastric cancer, and its mechanism of promoting gastric cancer development may involve up-regulating Skp2 expression and down-regulating RASSF1A expression.