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以葡萄品种‘左优红’组培苗叶片为材料,利用同源克隆法获得其病程相关蛋白1基因VvPR1的cDNA全长序列。扩增片段大小为486bp,编码161个氨基酸,分子量17.5kDa,等电点PI=8.69,含有6个保守半胱氨酸,4个allergenV5/Tpx-1related保守结构域。VvPR1与多种植物PR1高度同源。实时定量PCR检测结果表明VvPR1在葡萄叶片中相对表达量最高;霜霉病菌、低温、盐和干旱胁迫均可显著诱导其表达;水杨酸、脱落酸、茉莉酸、一氧化氮、过氧化氢和硫化氢等亦可诱导其大量表达,据此推测,VvPR1参与了多种生物胁迫和非生物胁迫过程。
The full length cDNA sequence of VvPR1 gene was obtained by homologous cloning method using leaves of grapevine ’Zuyouhong’ tissue culture seedlings as materials. The amplified fragment was 486bp in size, encoding 161 amino acids with a molecular weight of 17.5 kDa and an isoelectric point PI of 8.69. It contained six conserved cysteines and four conserved domains of allergenV5 / Tpx-1related. VvPR1 is highly homologous to a variety of plant PR1. Real-time PCR results showed that VvPR1 had the highest relative expression level in grape leaves; downy mildew, low temperature, salt and drought stress induced its expression significantly; salicylic acid, abscisic acid, jasmonic acid, nitric oxide and hydrogen peroxide And hydrogen sulfide can also induce a large number of its expression, suggesting that VvPR1 involved in a variety of biotic and abiotic stress processes.