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心肌缺血45min,再灌注180min复制兔急性心肌缺血再灌注模型。分别观察了等容血液稀释、维拉帕米以及二者联合应用对兔缺血再灌注心肌MDA含量、SOD活性及心功能的影响。实验并分四组:Ⅰ组(对照组);Ⅱ组(稀释组);Ⅲ组(维拉帕米组);Ⅳ组(稀释+维拉帕米组)。结果:①心肌组织MDA含量及SOD活性变化:与Ⅰ组缺血区心肌MDA含量相比,Ⅱ、Ⅲ、Ⅳ组均显著降低,Ⅳ组又明显低于Ⅱ、Ⅲ组;Ⅱ、Ⅲ、Ⅳ组缺血区心肌SOD活性均明显高于Ⅰ组(均P<0.05)。②心功能变化:再灌注后,Ⅰ组心功能进行性恶化,Ⅱ组左室收缩功能(LVSP,+dp/dt-max)和舒张功能(-dp/dt-max)有明显改善,Ⅲ组舒张功能明显改善,Ⅳ组各项心功能指标进一步好于Ⅱ、Ⅲ组(均P<0.05)。提示:等容血液稀释和维拉帕米均能保护缺血再灌注心肌的代谢和功能,二者合用效果更加明显.表现出良好的协同效应。
Myocardial ischemia 45min, 180min after reperfusion rabbit model of acute myocardial ischemia-reperfusion. The effects of isovolemic hemodilution, verapamil and their combination on MDA content, SOD activity and cardiac function in ischemia-reperfusion myocardium were observed. The experiment was divided into four groups: group Ⅰ (control group); group Ⅱ (dilution group); group Ⅲ (verapamil group); group Ⅳ (dilution + verapamil group). Results: ① The changes of MDA content and SOD activity in myocardium: Compared with those in group Ⅰ, the levels of MDA in group Ⅱ, Ⅲ and Ⅳ were significantly lower than those in group Ⅱ, while those in group Ⅳ were significantly lower than those in groups Ⅱ and Ⅲ The activity of SOD in ischemia group was significantly higher than that in group Ⅰ (all P <0.05). Cardiac function changes: After reperfusion, the cardiac function in group Ⅰ was deteriorated. The LVSP, dp / dt-max and diastolic function (-dp / dt-max) were significantly improved in group Ⅱ, Function improved significantly, each group Ⅳ heart function index is better than Ⅱ, Ⅲ group (all P <0.05). Hint: Isotonic hemodilution and verapamil both protect the metabolism and function of ischemic-reperfused myocardium, and the combined effect is more obvious. Show a good synergistic effect.