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目的研究硬紫草羟基萘醌提取物对脂多糖(LPS)诱导的小鼠腹腔巨噬细胞RAW264.7细胞炎症因子的抑制作用和对苯磺酸(TNBS)诱导的炎症性肠病(IBD)大鼠结肠组织中肿瘤坏死因子-α(TNF-α)表达的影响。方法对硬紫草羟基萘醌提取物进行提取分离,采用ELISA法测定硬紫草羟基萘醌提取物对LPS诱导的RAW264.7细胞释放的TNF-α和白细胞介素-1β(IL-1β)的抑制作用;用TNBS法制备IBD大鼠模型,造模24 h后ig给药,连续给药7 d,于末次给药24 h后处死大鼠,采用免疫组织化学法检测大鼠结肠组织中TNF-α的表达。结果硬紫草羟基萘醌提取物对LPS诱导的RAW264.7细胞释放的TNF-α和IL-1β具有抑制作用;与模型组相比,硬紫草羟基萘醌提取物可降低TNBS诱导的IBD大鼠结肠组织中TNF-α的表达。结论硬紫草羟基萘醌提取物对TNF-α和IL-1β炎症因子具有不同程度的抑制作用,并可抑制TNBS诱导的肠道炎症反应,这种抑制作用是通过降低TNF-α的表达来实现的。
OBJECTIVE To study the inhibitory effect of hardnitoside on the inflammatory factors induced by lipopolysaccharide (LPS) in mouse peritoneal macrophages RAW264.7 cells and on the inflammatory bowel disease (IBD) induced by benzenesulfonic acid (TNBS) Effect of Tumor Necrosis Factor-α (TNF-α) Expression in Colon Tissue of Rats. Methods Extraction and separation of hardnantherahydroxynaphthoquinone was carried out. The contents of TNF-α and IL-1β released by LPS-induced RAW264.7 cells were determined by ELISA. IBD rat model was established by TNBS. After 24 h, the rats were dosed with ig administration for 7 days. The rats were sacrificed 24 h after the last administration. Immunohistochemistry TNF-α expression. Results The extract of hardycin on hydroxynaphthoquinone could inhibit the release of TNF-α and IL-1β from LPS-induced RAW264.7 cells. Compared with the model group, the extract of hardycin on hydroxynaphthoquinone could reduce the TNBS-induced IBD Expression of TNF-α in rat colon tissue. Conclusions The extract of hardythin and hydroxynaphthoquinone can inhibit the TNF-α and IL-1β inflammatory cytokines to varying degrees and inhibit the TNBS-induced inflammatory reaction of the intestinal tract by decreasing the expression of TNF-α Achieve.