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1999～2001年连续2年,对 CIMMYT 提供的165份小麦品种进行成株期田间人工诱发抗白粉病鉴定。鉴定病圃设在本院试验农场,面积为200m~2。人工诱发鉴定圃的接种菌源,以上年度采集并保存的当地白粉病菌菌株,先在温室的感病品种8017-2上繁殖,2月下旬将带有大量分生孢子堆的病株移植到病圃的二个诱发行中间,每距33cm 种植2株病株。由于病株上有大量分生孢子,使诱发行麦株迅速受侵染且严重发病,形成充分接种菌源,保证对供鉴品种的重复侵染,以诱发供试小麦材料成株期感染发病。
From 1999 to 2001, for two consecutive years, 165 wheat cultivars from CIMMYT were identified for resistance to powdery mildew in adult field. Identification of nurseries located in our hospital test farm, an area of 200m ~ 2. Artificially induced identification of nursery inoculation source, the local collection and preservation of powdery mildew strains last year, first in the greenhouse susceptible varieties 8017-2 on the breeding, in late February with a large number of conidia infected plants transplanted into the disease In the middle of two induced lines of nursery, two diseased plants were planted at 33 cm intervals. Due to a large number of conidia on the diseased plants, the induced lines of wheat plants were rapidly infected and severely morbid, resulting in full inoculation of bacterial sources, ensuring repeated infection of the refeeding varieties and inducing the onset of adult plant infection of the tested wheat materials .