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目的 :了解紫杉醇联合直线加速器X 线放射对人喉鳞状细胞癌细胞株Hep 2的作用 ,为两种方法的临床联合运用提供实验依据。方法 :运用体外肿瘤细胞培养技术 ,观察 1× 10 -8mol/L紫杉醇联合直线加速器产生的 2GyX 线对人喉鳞癌细胞株Hep 2的生长抑制作用及时间效应。计算细胞存活率。结果 :加紫杉醇后放射组细胞生长明显抑制 ,第 7天时细胞存活率为 11.11% ,较放射后加紫杉醇组低。加紫杉醇 12h后放射与加紫杉醇 2 4h后放射其结果无明显差异。单纯紫杉醇组细胞存活率较单纯放射组低。结论 :紫杉醇联合直线加速器X 线放射明显抑制人喉鳞癌细胞株Hep 2的生长 ,紫杉醇可起放疗增敏作用
OBJECTIVE: To investigate the effect of paclitaxel combined with linear accelerator X-ray on human laryngeal squamous cell carcinoma cell line Hep 2 and provide experimental evidence for the clinical combination of the two methods. Methods: The in vitro tumor cell culture technique was used to observe the growth inhibitory effect and time effect of 2GyX line produced by 1 × 10 -8 mol / L paclitaxel plus linac on human laryngeal squamous cell carcinoma cell line Hep 2. Cell viability was calculated. Results: After adding paclitaxel, the growth of the radiotherapy group was significantly inhibited. On the 7th day, the cell survival rate was 11.11%, which was lower than that of the radiotherapy group and the paclitaxel group. After adding paclitaxel 12h after radiotherapy and adding paclitaxel 24h after radiotherapy the results no significant difference. The cell survival rate of paclitaxel group was lower than that of radiotherapy alone group. Conclusion: Paclitaxel combined with linear accelerator radiotherapy can significantly inhibit the growth of human laryngeal squamous cell carcinoma cell line Hep 2 and paclitaxel can radiosensitize