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以湖北省远安县苟家垭镇窑河村（北纬３１°１３′，东经１１１°２′，海拔５２５～５４０米）作为长日低温环境，进行了实用型水稻光温敏核不育系的选择和鉴定研究。参试材料有光敏型不育系ＮＫ５８Ｓ，低温敏型核不育系培矮６４５、ＨＮ５Ｓ和高温敏型不育系Ｗ６１５４Ｓ５、Ｗ８０１３Ｓ。试验方法是进行分期播种、连续观测小穗自交不育度，并同步观测气温。早稻类型的品种４月上旬播种，７月中旬可以齐穗，中、晚稻品种４月下旬播种，８月中、下旬可以齐穗。高温敏型的不育系在该点无明显的不育期，自交不育度在８０％左右，低温敏型的不育系基本保持稳定的不育。光敏型的ＮＫ５８Ｓ，敏感期处在２４℃以上时表现不育，２４℃以下时表现基本可育。当温度在２４℃上下波动时，育性也随之出现波动。研究结果初步认为，该点作为长日低温选择环境是可行的。早稻类型的材料可在７月中旬和８月中、下旬选择和鉴定，中、晚稻类型的材料可在８月中、下旬选择和鉴定。
Selection and identification of a practical thermo-sensitive genie male sterile line for rice under long-term low temperature environment in Yaohe Village, Goujiaasuanyuan, Yuanan County, Hubei Province (31 ° 13 ’N, 111 ° 2’E, 525 ~ 540 meters above sea level) the study. The tested materials were photosensitive CMS line NK58S, low temperature-sensitive genic male sterile line Pei’ai 645, HN5S and high temperature-sensitive male sterile lines W6154S5 and W8013S. The test method is to carry out sowing in phases, continuously observe the selfing sterility of spikelet and observe the temperature synchronously. Early varieties of rice varieties in early April sowing, mid-July can be full, medium and late rice varieties sowing in late April, mid-August, mid-to-full. The high temperature-sensitive sterile lines had no obvious sterile period at this point, the self-fertility was about 80%, and the low temperature-sensitive sterile lines basically remained stable sterile. Photosensitive NK58S, the sensitive period at 24 ℃ above the performance of sterile, below 24 ℃ when the performance of basic fertile. Fertility also fluctuates when the temperature fluctuates around 24 ° C. The preliminary results suggest that this point is feasible as a long-term low temperature environment. Materials of early rice type can be selected and appraised in mid-July and the middle of August, and materials of medium-late rice type can be selected and appraised in middle and late August.